Lab Technician I

Company: Unicon Pharma
Location: Hartford
Posted on: May 21, 2020

Job Description:

Job Description: This job function will support large scale cell culture work and reagent generation in Structural Biology and Biophysics. This position will be physically intensive requiring the ability to lift and move large, heavy flasks. Duties may include, but are not limited to: Prepare large scale cell reagents using mammalian cells, E.coli and baculovirus with insect cells. Propagate and maintain various continuous cell lines. Verify the protein expression using various protocols including flow cytometry, Western Blots, and small scale purification. Experimental data will be recorded in a provided electronic notebook according to the guidelines. Responsible for ordering supplies as needed to deliver cell and protein reagents. As time permits, support lab with generation of various solutions and some molecular biology including mutagenesis and large scale plasmid production. Coordination with various groups on delivery time-line of cell pastes. Job Requirements: BS in biology or related field with 0-2 years of relevant experience in mammalian and microbial culture techniques including cell expansion and cryopreservation, protein expression optimization and scale-up, protein expression analysis by SDS-PAGE and Western Blot Methods. Excellent aseptic technique is required. Experience using standard laboratory equipment including incubators, microscopes, pipettes, and biosafety cabinets. Ability to effectively supply a wide array of cell reagents on a regular basis to support weekly requests is critical. Ability to quickly identify and remedy potential issues with cell culture. Ability to keep accurate records using electronic notebooks and create common laboratory reagents including buffers and cell culture media. Must be able to work on multiple projects at one time at a time and have a positive customer service focus. Computer skills required: Microsoft Word, Excel, and Outlook. Ability to work in a high-throughput environment, stay organized, and to adhere to specific timelines. Team player with attention to detail, and excellent written and verbal communication. Must be self-motivated, very reliable and have a strong attention to detail.

Keywords: Unicon Pharma, Hartford , Lab Technician I, Healthcare , Hartford, Connecticut